HIV-1 NL4-3-HA 腹腔途径感染NOD小鼠模型

来源文献：Humanized Mouse Model of HIV-1 Latency with Enrichment of Latent Virus in PD-1 + and TIGIT + CD4 T Cells

PMID:30842333

DOI：10.1128/JVI.02086-18

NOD.Cg-Prkcdscid Il2rgtm1Wjl/SzJ (NSG) neonatal mice were sub-lethally irradiated and injected with 1 x 10^6 human fetal liver CD34+ cells per mouse. Nine humanized mice were infected with the HIV-1 strain NL4-3-HA (47) for 10 weeks, and then 4 were switched to ART-containing feed. Humanized mice between ages 12-20 weeks, engrafted with at least 30% human CD45+ cells in blood, of which at least 10% were CD4+, were infected via intraperitoneal injections containing 5-10 x 10^5 infectious units of HIV-1. This formulation was calculated by consideration of the average amount of food consumed by the mice (3.5g per day) and their average weight (20g) and provided average daily drug doses of Emtricitabine (FTC) at 87.5mg/kg/day, Tenofovir (TDF) at 131mg/kg/day, and Raltegravir (RAL) and Darunavir (DRV) at 175mg/kg/day each.

诊断：病毒在血液中复制。

所有感染动物必须在ABSL-3室中进行。

1.该模型制备比较成熟，国内外都有此模型，构建和评价指标一致，所以该模型应用广泛，具有统一的操作规程。

2. 该模型制备的关键：1）小鼠必须是重度免疫缺陷的，而且饲养环境必须洁净；2）HIV操作需要生物安全。

3.此模型具有完善的人的免疫系统，HIV感染后，具有艾滋病病人相似的临床症状，所以具有很高的比较医学应用价值。