小动物烫伤模型前列腺素检测

信息概要

• 小动物烫伤模型前列腺素检测是一种专门用于评估烫伤后炎症反应的检测服务，通过测量前列腺素水平来深入了解烫伤引起的病理变化。该检测对于药物研发、治疗效果评估和机制研究具有重要意义，帮助研究人员量化炎症介质，为临床前研究提供关键数据。

检测项目

• PGE2
• PGF2α
• PGD2
• PGI2
• TXA2
• TXB2
• 6-keto-PGF1α
• 13,14-dihydro-15-keto-PGE2
• 13,14-dihydro-15-keto-PGF2α
• PGE1
• PGF1α
• PGA2
• PGB2
• PGC2
• PGD3
• PGE3
• PGF3α
• 11β-PGF2α
• 15-keto-PGE2
• 15-keto-PGF2α
• 8-iso-PGF2α
• 2,3-dinor-8-iso-PGF2α
• 2,3-dinor-TXB2
• 11-dehydro-TXB2
• COX-1 activity
• COX-2 activity
• Prostaglandin E synthase activity
• Prostaglandin F synthase activity
• Thromboxane synthase activity
• Prostacyclin synthase activity

检测范围

• PGE series
• PGF series
• PGD series
• PGI series
• TXA series
• Metabolites of PGE2
• Metabolites of PGF2α
• Metabolites of PGD2
• Metabolites of PGI2
• Metabolites of TXA2
• Isoprostanes
• Dihydroketo metabolites
• Dinor metabolites
• Dehydro metabolites
• COX-derived prostaglandins
• Lipoxygenase-derived mediators
• Cyclooxygenase products
• Prostanoids
• Eicosanoids
• Oxylipins
• Inflammatory mediators
• Bioactive lipids
• Signal molecules
• Tissue hormones
• Autacoids
• Vasoactive substances
• Pain mediators
• Fever inducters
• Immune modulators
• Cell proliferation regulators

检测方法

• Enzyme-Linked Immunosorbent Assay (ELISA) - A plate-based assay using antibodies for specific prostaglandin detection.
• High-Performance Liquid Chromatography (HPLC) - Separates prostaglandins based on chemical properties for quantification.
• Liquid Chromatography-Mass Spectrometry (LC-MS) - Combines separation with mass detection for accurate analysis.
• Gas Chromatography-Mass Spectrometry (GC-MS) - Used for volatile prostaglandin derivatives after chemical treatment.
• Radioimmunoassay (RIA) - Employs radioactive labels to measure prostaglandin concentrations.
• Immunofluorescence - Detects prostaglandins in samples using fluorescent-labeled antibodies.
• Western Blot - Adapted for detecting prostaglandin synthases and related proteins.
• Spectrophotometry - Measures absorbance changes in prostaglandin assays.
• Fluorometry - Utilizes fluorescence for sensitive prostaglandin detection.
• Thin-Layer Chromatography (TLC) - A simple method for prostaglandin separation and identification.
• Ultra-Performance Liquid Chromatography (UPLC) - Provides faster and higher resolution separation than HPLC.
• Tandem Mass Spectrometry (MS/MS) - Offers high specificity for prostaglandin identification and quantification.
• Enzyme Immunoassay (EIA) - Similar to ELISA, using enzyme reactions for detection.
• Chemiluminescence Immunoassay - Relies on light emission for high-sensitivity prostaglandin measurement.
• Immunohistochemistry - Visualizes prostaglandin distribution in tissue sections.
• Flow Cytometry - Analyzes cell-based prostaglandin production in suspensions.
• Polymerase Chain Reaction (PCR) - Detects gene expression of prostaglandin synthases.
• Microarray Technology - Allows high-throughput screening of prostaglandin-related genes.
• Biosensor Assays - Uses biological elements for real-time prostaglandin detection.
• Capillary Electrophoresis - Separates prostaglandins based on charge and size in capillaries.

检测仪器

• HPLC System
• LC-MS System
• GC-MS System
• ELISA Reader
• Microplate Reader
• Spectrophotometer
• Fluorometer
• Centrifuge
• Incubator
• Autosampler
• Mass Spectrometer
• Chromatography Column
• Pipettes
• Western Blot Apparatus
• PCR Machine
• Flow Cytometer
• Microarray Scanner
• Biosensor Device
• Capillary Electrophoresis System
• TLC Plate