HIV-1 JR-CSF腹腔途径感染人源化NOG-hCD34小鼠模型

来源文献：Dynamics and mechanisms of clonal expansion of HIV-1-infected cells in a humanized mouse model

PMID：28761140

DOI：10.1038/s41598-017-07307-4

We transplanted human CD34+ hematopoietic stem cells into the liver of new-born recipient NOD/SCID/Il2rgnull mice (NOG mice). Afer 10–12 weeks post-transplantation, the humanized mice (n=6) were intraperitoneally infected with the CCR5-tropic HIV-1JR-CSF. We divided the six mice into two groups; respectively 2 weeks post infection (wpi) and 15 wpiNOD/SCID/Il2rgnull mice (NOG mice) were obtained from the Central Institute for Experimental Animals (Kawasaki, Japan)39. Human CD34+ hematopoietic stem cells were isolated from human fetal liver kindly provided by Dr. Dong Sung An (University of Los Angeles, USA). We generated humanized mice (NOG-hCD34 mice) as described previously40, 41. HIV-1JR-CSF viral solution was prepared as previously described42. Briefy, pJR-CSF, an infectious molecular clone of HIV-1JR-CSF43, 44, was transfected into 293T cells. At 48 h post-transfection, the culture supernatant was harvested, centrifuged, and then fltered through a 0.45-μm-pore-size flter to produce virus solutions. Viral solution (1,500 infectious units [IU]) was intraperitoneally injected into the NOG-hCD34 mice.

诊断：病毒在血液中复制。

所有感染动物必须在ABSL-3室中进行。

1.该模型制备比较成熟，国内外都有此模型，构建和评价指标一致，所以该模型应用广泛，具有统一的操作规程。

2. 该模型制备的关键：1）小鼠必须是重度免疫缺陷的，而且饲养环境必须洁净；2）HIV操作需要生物安全。

3.此模型具有完善的人的免疫系统，HIV感染后，具有艾滋病病人相似的临床症状，所以具有很高的比较医学应用价值。