HIV-1 JRCSF腹腔途径感染NSG小鼠模型

来源文献：Minocycline attenuates HIV-1 infection and suppresses chronic immune activation in humanized NOD/LtsZ-scidIL-2Rγ(null) mice.

PMID:24409837

DOI:10.1111/imm.12246

We transplanted 2 9 105 freshly isolated CD34+ cells into mice by intravenous tail injection within 16–24 hr of a second busulfan injection. After 130–150 days of CB-HPC transplantation, nine groups of mice (n = 70) (Table 1) were inoculated with HIV- 1JRCSF (5000 TCID50) by intraperitoneal route. Group 5, 6, 7 and 8 mice were treated with minocycline at a dose of 100 mg/kg/day by intraperitoneal injection. Six mice of group 9 were used to measure the basal level of cytokines and LPS in the plasma samples.

诊断：病毒在血液中复制。

所有感染动物必须在ABSL-3室中进行。

1.该模型制备比较成熟，国内外都有此模型，构建和评价指标一致，所以该模型应用广泛，具有统一的操作规程。

2. 该模型制备的关键：1）小鼠必须是重度免疫缺陷的，而且饲养环境必须洁净；2）HIV操作需要生物安全。

3.此模型具有完善的人的免疫系统，HIV感染后，具有艾滋病病人相似的临床症状，所以具有很高的比较医学应用价值。