HIV-1 ADA腹腔途径感染CD34-NSG小鼠模型

来源文献：Human Interleukin-34 facilitates microglia-like cell differentiation and persistent HIV-1 infection in humanized mice

PMID：30832693

DOI：10.1186/s13024-019-0311-y

Mice with established human hemato-lymphoid reconstitution (∼6–8 months of age) and positive for IL-34 expression were intraperitoneally infected (n =12) with the 104 tissue culture infectious dose50 (TCID50) of macrophage-tropic HIV-1ADA strain.NOG (formally, NOD.Cg-Prkdcscidil2gtmlSug/Jic) and NOD/ShiJcl (NOD) were used. NOG mice were maintained in the CIEA under specific-pathogen-free conditions. NOD mice were purchased from CIEA (Tokyo,Japan). To generate the hIL-34 expressing transgenic NOG mouse (NOG-hIL-34), a linearized DNA vector (pCMV6-XL4) containing human IL-34 cDNA (Origene Technologies, Inc., Rockville, MD, USA) under the control of a CMV promoter, was microinjected into fertilized eggs obtained by mating NOG and NOD mice.

诊断：病毒在血液中复制。

所有感染动物必须在ABSL-3室中进行。

1.该模型制备比较成熟，国内外都有此模型，构建和评价指标一致，所以该模型应用广泛，具有统一的操作规程。

2. 该模型制备的关键：1）小鼠必须是重度免疫缺陷的，而且饲养环境必须洁净；2）HIV操作需要生物安全。

3.此模型具有完善的人的免疫系统，HIV感染后，具有艾滋病病人相似的临床症状，所以具有很高的比较医学应用价值。