HIV CCR5-tropic静脉途径感染人源化BLT小鼠模型

来源文献：Generation of HIV latency in humanized BLT mice.

PMID：22013053

DOI:10.1128/JVI.06120-11

Briefly, preconditioned NOD/SCID-gamma chain null (NSG) mice (The Jackson Laboratories) implanted with thymus and liver tissue (Advanced Bioscience Resources) were transplanted with 2×10^5 to 3×10^5 autologous human fetal liver CD34+ cells and monitored for human engraftment.Humanized BLT mice are susceptible to vaginal, rectal, and intravenous HIV infection (10– 12, 36). We infected BLT mice with CCR5-tropic HIV (JR-CSF or SF162). Infected BLT mice received ART consisting of daily intraperitoneal injections of emtricitabine (FTC; 140 to 200 mg/kg body weight), tenofovir disoproxil fumarate (TDF; 146 to 208 mg/kg), and raltegravir (RAL; 56 to 80 mg/kg). BLT mouse that received ART for 58 days (followed for a total of 77 days postexposure) as well as in a pair of untreated, infected BLT mice (followed for a total of 69 and 70 days postexposure).

诊断：血浆病毒血症。

所有感染动物必须在ABSL-3室中进行。

1.该模型制备比较成熟，国内外都有此模型，构建和评价指标一致，所以该模型应用广泛，具有统一的操作规程。

2. 该模型制备的关键：1）小鼠必须是重度免疫缺陷的，而且饲养环境必须洁净；2）HIV操作需要生物安全。

3.此模型具有完善的人的免疫系统，HIV感染后，具有艾滋病病人相似的临床症状，所以具有很高的比较医学应用价值。