首页检测项目新闻动态搜索一下

我们的检测流程严格遵循国际标准和规范，确保结果的准确性和可靠性。我们的实验室设施精密完备，配备了最新的仪器设备和领先的分析测试方法。无论是样品采集、样品处理还是数据分析，我们都严格把控每个环节，以确保客户获得真实可信的检测结果。

咨询报价

SARS-CoV Urbani 气管滴鼻联合感染雪貂模型

原创版权来源：中析研究所发布时间：2023-03-20 04:06:14 点击：【大中小】 | 【打印】

阅读不方便？点击直接咨询工程师！

全国服务领域：河北、山西、黑龙江、吉林、辽宁、江苏、浙江、安徽、福建、江西、山东、河南、湖北、湖南、广东、海南、四川、贵州、云南、陕西、甘肃、青海、台湾、内蒙古、广西、西藏、宁夏、新疆、北京、天津、上海、重庆、香港、澳门

模型信息

中文名称:SARS-CoV Urbani 气管滴鼻联合感染雪貂模型

英文名称:SARS-CoV Urbani intratracheally and intranasally infected ferret model

类型:SARS-CoV感染动物模型

分级:NA

用途:用于SARS-CoV感染研究。

研制单位:美国食品药品管理局@@https://www.fda.gov

保存单位:美国食品药品管理局@@https://www.fda.gov

研究背景

暂无介绍

制备方法

To generate the vaccine, 5.94 mL of virus stock ( 1*10^5 TCID50/mL)was treated with 60 mL of 3.7% formalin for 48 h at 37 °C. The inactivated virus (6 mL) was then dialyzed in 2 L of PBS at room temperature for 48h. Medium from uninfected Vero cells was treated in the same way to prepare mock vaccine.Vaccine was plated on Vero cells for 7 days to confirm inactivation. Vaccination was done intramuscularly at 0.5 mL per leg without adjuvant. The virus challenge ( 1x10^5 TCID50/mL) was split into 2 parts: 0.25 mL was given intratracheally, and 0.25 mL was given intranasally. A laryngoscope was used for the intratracheal portion, which was delivered via a 1-cc syringe with a 22-gauge plastic angiocatheter attached. The intranasal inoculum was delivered to each nostril via a plastic bulb–type pipettor.