SARS-CoV PUMC01 滴鼻感染野生小鼠模型
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模型信息
中文名称:SARS-CoV PUMC01 滴鼻感染野生小鼠模型
英文名称:SARS-CoV PUMC01 intranasally infected wild-type mice model
类型:SARS-CoV感染动物模型
分级:NA
用途:用于SARS-CoV感染研究。
研制单位:北京协和医院@@https://www.pumch.cn/en.html
保存单位:北京协和医院@@https://www.pumch.cn/en.html
研究背景
暂无介绍
制备方法
hACE2 cDNA was cloned as previously described35 and inserted into the pEGFP-N1 plasmid (BD Biosciences) upstream of the SV40 polyA tract, replacing the coding sequence for enhanced green fl uorescence protein. We retrieved the mouse ACE2 promoter (9 kilobases) by digesting bacterial artifi cial chromosome (RP23-75P20) DNA with KpnI and EcoRV and inserted it upstream of the hACE2 coding sequences. After hACE2 was identifi ed in transfected NIH3T3 cells, the plasmid was linearized with KpnI and KasI and purifi ed with phenol. Fragments carrying the mouse ACE2 promoter driving the hACE2 coding sequence were introduced by microinjection into the pronuclei of fertilized ova from ICR mice. The injected zygotes then were transplanted into the oviducts of pseudopregnant mice. Mice in which the transgene integrated were identifi ed from tail biopsies. These mice were bred to produce offspring for analysis.
评价验证
暂无介绍
生物安全性
暂无介绍
讨论与结论
暂无介绍
注意：因业务调整，暂不接受个人委托测试。
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